Student Research Projects

Determination of Creatine Excretion Before and After Strenuous Activity by HPLC

Student Darren Driscoll ‘14
Faculty Mentor(s)
Department Chemistry
Course Chemistry 480: Instrumental Methods of Analysis


The purpose of this project was to analyze urine for creatinine before and after physical activity by collegiate athletes. Creatine plays a major role in the muscle’s ability to perform and maintain short duration, high intensity exercise. Once the activity is over, the creatine is spontaneously converted to creatinine, becomes wasteful, and is excreted from the body. These results could be used to determine whether specific athletes are maximizing the energy benefits of a difficult workout through creatinine levels found in the urine. Creatinine levels were determined by High Pressure Liquid Chromatography (HPLC) using a 85:15 Water:Acetonitrile mobile phase. Preliminary results indicate that creatinine levels are identifiable in HPLC.


Selected as best research project in the Division of the Natural Sciences and Mathematics at VWC's annual 2014 Undergraduate Research Symposium, Division of the Humanities